Biology project: bacterial beginnings!
Jan 16th, 2007 by lapazfarm
Starting off the new year here at Lapaz Farm with a renewed commitment to doing what we do best…projects!!!!We’ve set up our new school areas with hands-on learning in mind. We have work stations for every subject! A nature study center, a geo center (for geology and geography) a math lab, a living history corner, and a language lab! (We have yet to set up our home altar for religious studies, but it’s coming!)
To kick it off, we have finally begun our big biology project I had planned way back when. We began by learning about the 5 kingdoms (and other approaches such as 8 kingdoms, 3 domains, etc…. Superboy learned the basic shapes of bacteria with his cards and definitions. We learned about harmful and beneficial bacteria and then made a batch of yogurt to test out our knowledge, using the new yogurt maker I got for Christmas. Yummy!It turned out fabulous!
Then we had a lot of fun with this bacteria experiment:
Superboy investigated which disinfectant worked the best to kill bacteria. He melted agar and poured 10 plates (aka petri dishes) and let them cool. He then made a broth with water, some yogurt, and some soil, in hopes of getting a variety of bacteria types. He put 9 ml of the broth into cups and added 1 ml of each disinfectant into the cups.
He used the following disinfectants:
Rubbing Alcohol
Iodine
Dial Soap
bleach (10% solution)
disinfectant wipes (store brand, squeezed the liquid from them)
Dawn dishwashing liquid
Swirl to mix. Then he innoculated the plates with each of the disinfected mixtures and l plate with the plain broth as a control. Let incubate for 5 days in a warm dark place.

Results were a bit surprising! Compared to the control, the alcohol, Iodine, and wipes did little to disinfect the broth. The dial soap and the bleach were the clear winners, with the Dawn soap in third(much of what you see in the Dawn plate is just condensation in the dish). We looked at some samples under our microscope and saw the expected bacillus from the yogurt, plus many, many dancing, swirling cyanobacteria from the soil! They sure are active little beasties! What a difference to observe them live rather than from pictures or prepared slides!
Then for fun we decided to investigate the wives tale that a dog’s mouth is cleaner than a human’s.
We swabbed Superboy’s mouth (pre-toothbrushing for fairness), our Golden retriever Buddy’s mouth, and left one plate clean as a control.
Results:

Superboy had a few more spots, but Buddy’s entire plate is covered with a thin white film of growth. Whose mouth is cleaner? You be the judge!
More fun tomorrow! And I’ll tell you about the other projects he has going as well!

This is awesome. I’m so impressed with the way you and your children dive into science.
Two thumbs up on this project! Awesome!
I am sooo envious of your work stations…
Your projects look SO FUN!!! How powerful does the microscope have to be to see the live bacteria. We have one, but I don’t know if it would be powerful enough. I want to do that experiment so bad. We have talked about Bacteria, and I would love to be able to follow up with that experiment…help me!!!
I vote on the dog’s mouth being more unclean. I would much rather smell a little boy’s breath than a dogs!!! Plus, a little boy doesn’t lick his bottom!!! That’s all the evidence that I need…hahaha!
Way to go!!! You are awsome!
I LOVE SCIENCE!!!
Glad you’re back! Your biology study is blossoming beautifully.
Sherri
Our microscope goes to 400X and the bacteria are visible but quite small. But even if yours is not as powerful, don’t let that dissuade you from trying this project. Really, looking at the bacteria under the scope was a small part of it. The plates were fascinating even without that added activity. I say go for it!
Looks lik a lot of fun – we dove into some Science yesterday, too!
This looks really cool! Can you recommend a mail-order source for agar, by chance?
I love your site! I just found it today. In fact, we were studying “germs” this week and talking about bacteria and viruses. Because of your blog, I just ordered a kit from Home Science Tools. We’re going to grow some bacteria!
Thanks & please keep it up. Your kids are learning so much, and we appreciate you sharing your ideas!!!
Dana
Thanks, Dana! I’m lad you are enjoying the blog. I browsed through yours and it looks like you are doing some really fun stuff with your daughter!
Kris, I got the whole kit from home science tools, agar included. I like the melt and pour agar myself, it’s much easier than the powdered stuff. They have a pretty nice kit. We didn’t follow the directions in the kit, but they were good.